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Substrate solution is then added and color develops in proportion to the amount of Human TJP2
only those wells that contain Human EFNA1
And then biotinylated Mouse ANKRD55 Antibody is added and binds to ANKRD55 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated MAP1LC3B antibody
Human SPAG5 (sperm associated antigen 5) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human SPAG5 (sperm associated antigen 5) ELISA Kit measures SPAG5 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SPAG5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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